Author:

Keywords:

Action Potentials, Animals, Electrophysiology, Heart, Heart Atria, Heart Ventricles, Male, Membrane Potentials, Myocardial Contraction, Phenylephrine, Rats, Rats, Inbred Strains, Research Support, Non-U.S. Gov't, Science & Technology, Life Sciences & Biomedicine, Pharmacology & Pharmacy, PHENYLEPHRINE, ACTION POTENTIAL, RESTING MEMBRANE POTENTIAL, K+ CURRENT, CA2+ CURRENT, NA+/K+ PUMP, TRANSIENT OUTWARD CURRENT, ALPHA-ADRENOCEPTOR STIMULATION, PROTEIN-KINASE-C, SARCOPLASMIC-RETICULUM, INOSITOL TRISPHOSPHATE, PAPILLARY-MUSCLE, MYOCYTES, CELLS, CA-2+, ALPHA-1-ADRENOCEPTORS, 1115 Pharmacology and Pharmaceutical Sciences, 1116 Medical Physiology, 3214 Pharmacology and pharmaceutical sciences

Abstract:

Stimulation of alpha 1-adrenoceptors evokes a different pattern of inotropic responses in atrial and ventricular heart muscle preparations from rats. The inotropic effects are accompanied by different changes in membrane potential. In an attempt to clarify the question whether or to which extent these events are causally related, the effects of phenylephrine on force of contraction, transmembrane potential, Ca2+ current (ICa) and K+ currents were comparatively studied in either tissue. In atrial preparations, phenylephrine 10 mumol/l caused an increase in force of contraction, a marked prolongation of the action potential duration and a depolarization of the membrane at rest. In the ventricle, however, the addition of phenylephrine 10 mumol/l produced first a decline in force of contraction associated with a hyperpolarization of the membrane and a reduction in the action potential duration. These changes were followed by an increase in force of contraction and a slight prolongation of the action potential, whereas the resting membrane potential remained increased. The hyperpolarization was eliminated in the presence of ouabain 100 mumol/l. In enzymatically isolated atrial and ventricular myocytes, the whole-cell voltage clamp technique was used to study membrane currents on exposure to phenylephrine. Phenylephrine 30 mumol/l did not affect the magnitude of ICa in either cell type. Transient and steady state K+ outward currents, however, were significantly diminished to a similar extent in atrial and in ventricular myocytes. It is concluded that the positive inotropic effect of alpha 1-adrenoceptor stimulation in the rat atrium is related to an increase in action potential duration and a decrease in resting membrane potential due to a decrease in K+ currents.(ABSTRACT TRUNCATED AT 250 WORDS)