Title: Free energy of ATP-hydrolysis fails to affect ATP-dependent potassium channels in isolated mouse ventricular cells
Authors: Albitz, R ×
Kammermeier, H
Nilius, Bernd #
Issue Date: May-1990
Series Title: Journal of Molecular and Cellular Cardiology. vol:22 issue:2 pages:183-90
Abstract: The single channel recording technique has been used to study the adenosine-5'-triphosphate (ATP)-dependent K+ channel in isolated mouse ventricular cells. The aim of this work was to determine if the activity of the K+ channel depends on the free energy of ATP-hydrolytic reaction (phosphorylation potential) in addition to the well-studied direct block by ATP. When the phosphorylation potential was changed from 60 to 50, to 40 and back to 60 kJ/mol at a constant ATP-concentration of 10(-4) mol/l, the ATP-channel activity showed a run down that is best described by a linear function. Changing the ATP-concentration of the bath solution from 0.01 to 1 and back to 0.01 mmol/l by a factor of 10 at a constant phosphorylation potential of 50 kJ/mol, resulted in a run down of the mean average current by a reduction of the open state probability in a concentration dependent manner. A dissociation constant of about 0.14 mmol/l could be estimated. The amplitude of the single channel current was not affected by ATP-concentrations in contrast to changes in phosphorylation potential. A significant increase in the single channel current accompanying a decrease in the phosphorylation potential at constant ATP concentrations was observed. This effect might be due to a decrease of free Mg2(+)-concentration by an increase of the ADP concentration in solutions with lower phosphorylation potentials. An allosteric regulation of the ATP-dependent K+ channel dependent on the free ATP concentration together with the Mg2(+)-ADP concentration seems to be a more likely explanation than regulation by phosphorylation/dephosphorylation secondary to a breakdown of ATP.
ISSN: 0022-2828
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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