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Title: Intra- and intercellular Ca2+ signaling in retinal pigment epithelial cells during mechanical stimulation
Authors: Himpens, Bernard ×
Stalmans, Peter
Gomez, P
Malfait, M
Vereecke, Johan #
Issue Date: Jun-1999
Publisher: The Federation of American Societies for Experimental Biology
Series Title: FASEB Journal vol:13 Suppl pages:S63-8
Abstract: The intercellular communication (IC) was investigated between cultured rat retinal pigment epithelial (RPE) cells isolated from Long-Evans (LE) or dystrophic Royal College of Surgeons (RCS) rats and grown in solutions containing normal and high glucose concentrations, or after modulation of protein kinase C (PKC). This was performed by studying the conduction of the free Ca2+-concentration ([Ca2+]i) wave elicited by mechanical stimulation and by analyzing the fluorescence recovery after photobleaching (FRAP). Mechanical stimulation of LE-RPE cells triggers Ca2+ influx, mediated by stretch-sensitive cation channels followed by intracellular Ca2+ release. A regenerative [Ca2+]i wave was found with a lower propagation rate in RCS-RPE cells. This rate could be increased by PKC down-regulation. Mechanical stimulation caused a [Ca2+]i increase in the mechanically stimulated (MS) cell followed after a delay by a [Ca2+]i rise in the adjacent cell layers. The intercellular [Ca2+]i wave propagation could be blocked by gap junction blockers such as halothane or PKC activation. An inhibition of the [Ca2+]i-wave propagation similar to that induced by halothane could be observed in cells grown in solutions containing 14 mM or higher concentrations of glucose. PKC down-regulated cells grown in glucose-rich medium did not develop this inhibitory effect on gap junction communication (GJC). FRAP experiments confirmed that the observed changes were consistent with a PKC-mediated inhibitory effect of high glucose concentrations on GJC.
ISSN: 0892-6638
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular and Cellular Signaling
Research Group Ophthalmology
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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