Title: Isolation of calelectrin-like proteins associated with smooth muscle plasma membranes
Authors: Raeymaekers, Luc ×
Wuytack, Frank
Casteels, Rik #
Issue Date: Dec-1985
Series Title: Biochemical and Biophysical Research Communications vol:132 issue:2 pages:526-32
Abstract: Plasma membranes prepared from pig stomach smooth muscle (antral part) were extracted with Triton X-100 to isolate insoluble cytoskeletal components. Reextraction of the insoluble material in EGTA yielded a protein complex which resembled the family of proteins that has been designated as 'mammalian calelectrins' [Südhof, T.C. et al. (1984) Biochemistry 23, 1103-1109]. Plasma membranes prepared in the presence and in the absence of 0.6 M KCl differed by the amount of these proteins, but in both preparations the EGTA-extractable proteins were quantitatively important constituents. Two of these proteins were further purified by means of ion exchange chromatography to apparent homogeneity as judged from sodium dodecyl sulfate gel electrophoresis.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Laboratory of Cellular Transport Systems
× corresponding author
# (joint) last author

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