Journal Of Organic Chemistry
Author:
Keywords:
Science & Technology, Physical Sciences, Chemistry, Organic, Chemistry, BROAD SUBSTRATE-SPECIFICITY, ALCOHOL-DEHYDROGENASE, ENANTIOSELECTIVE REDUCTION, CARBONYL REDUCTASE, ASYMMETRIC REDUCTION, CHIRAL ALCOHOLS, OXIDATION, SYSTEM, TOOL, Alcohol Dehydrogenase, Catalysis, Escherichia coli, Ketones, Molecular Structure, Oxidation-Reduction, Ralstonia, Substrate Specificity, ras Proteins, 0304 Medicinal and Biomolecular Chemistry, 0305 Organic Chemistry, Organic Chemistry, 3404 Medicinal and biomolecular chemistry, 3405 Organic chemistry
Abstract:
Ketones with two bulky substituents, named bulky-bulky ketones, as well as less sterically demanding ketones were successfully reduced to the corresponding optically highly enriched alcohols using a novel identified recombinant short-chain alcohol dehydrogenase RasADH from Ralstonia sp. DSM 6428 overexpressed in E. coli.