Publication date: 2019-07
Volume: 35 Pages: 561 - 572
ISSN: 0892-7014, 1029-2454 PMID: 31282200
DOI: 10.1080/08927014.2019.1630386
Publisher: Taylor & Francis


Maquera Huacho, Patricia Milagros
Herrero, Esteban Rodriguez ; Verspecht, Tim ; Pauwels, Martine ; Marcantonio, Elcio ; Palomari Spolidorio, Denise Madalena ; Teughels, Wim


Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Marine & Freshwater Biology, Terpinen-4-ol, carvacrol, peri-implantitis, periodontal disease, multi-species biofilm, TEA TREE OIL, CHLORHEXIDINE DIGLUCONATE, ANTIMICROBIAL ACTIVITY, PERI-IMPLANT, MECHANISMS, SUSCEPTIBILITY, INFECTIONS, COMPONENTS, RESISTANCE, TOXICITY, Biofilms, Chlorhexidine, Cymenes, Humans, Monoterpenes, Terpenes, Marine Biology & Hydrobiology, 05 Environmental Sciences, 06 Biological Sciences, 10 Technology


The aim of this study was to investigate the cytotoxic activity and inhibitory effect of terpinen-4-ol (T4ol) and carvacrol against single- and multi-species biofilms. The toxicity of each compound was tested on oral keratinocytes and evaluated by XTT assay. Inhibition and eradication of single-species biofilms were analyzed by crystal violet assay and the effect on multi-species biofilm composition was evaluated by qPCR. T4ol and carvacrol did not affect the epithelial cell viability, in contrast to chlorhexidine, which showed a high cytotoxic effect. Inhibition and eradication of single-species biofilms treated with T4ol and carvacrol were observed. The same inhibitory effect was observed for multi-species biofilms, especially on periodontal pathogens. In conclusion, specific concentrations of T4ol and carvacrol without toxicity towards the epithelial cells reduced the numbers of periodontal pathogens in single- and multi-species biofilms.