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Applied and Environmental Microbiology

Publication date: 2013-02-01
Volume: 79 Pages: 3126 - 3128
Publisher: American Society for Microbiology (ASM)

Author:

Boons, Kathleen
Van Derlinden, Eva ; Mertens, Lauren ; David, Charlotte C ; Hofkens, Johan ; Van Impe, Jan

Keywords:

Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Microbiology, GEL MICROSTRUCTURE, LISTERIA-INNOCUA, GROWTH, Culture Media, Dextrans, Escherichia coli, Gelatin, Microscopy, Confocal, 3107 Microbiology, 3207 Medical microbiology

Abstract:

In a gelatin-dextran mixture, changing the (relative and/or absolute) concentration of the components leads to the formation of different microstructures. Confocal laser scanning microscopy illustrated that the nature of the microstructure determines the location and morphology of Escherichia coli colonies. Observations indicate that bacterial growth preferentially occurs in the dextran phase, regardless of the microstructure.