Download PDF (external access)

EMBO Journal

Publication date: 2005-12
Volume: 24 Pages: 4115 - 23
ISSN: 0261-4189, 1460-2075 PMID: 16281053
DOI: 10.1038/sj.emboj.7600871
Publisher: Nature Publishing Group

Author:

De Wever, Veerle
Reiter, Wolfgang ; Ballarini, Annalisa ; Ammerer, Gustav ; Brocard, Cécile

Keywords:

Adaptation, Physiological, DNA-Binding Proteins, Glucose, Nuclear Localization Signals, Oxidative Stress, Phosphoprotein Phosphatases, Phosphorylation, Protein Phosphatase 1, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Serine, Transcription Factors, Developmental Biology, 06 Biological Sciences, 08 Information and Computing Sciences, 11 Medical and Health Sciences

Abstract:

In yeast, glucose depletion elicits a quick response in the transcription of stress-related genes. The main transcriptional activator that orchestrates this response is Msn2, whose nuclear localization and DNA binding are negatively controlled by the cAMP-dependent protein kinase (PKA). Msn2 activation by sudden glucose depletion correlates with a fast but transient decrease in phosphorylation of several sites in its nuclear localization signal (NLS). Here we show that protein phosphatase 1 (PP1) is the direct antagonist of PKA-dependent phosphorylation at the Msn2 nuclear import domain and therefore a potential mediator of glucose starvation signals that target this transcription factor. Apart from PKA, the protein kinase Snf1 can also directly modify one of the Msn2 phosphorylation sites (S582) and thereby repress Msn2 function. Consequently, in snf1 mutants, rephosphorylation of the NLS happens to be much slower during prolonged starvation. Thus, a second, Reg1-dependent form of PP1 indirectly influences Msn2 functionality by modulating Snf1 kinase activation and repression. Different activities of PP1 are therefore involved in shaping induction and adaptation of the transcriptional stress response during acute glucose starvation.