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Methods in Molecular Biology

Publication date: 2009-01
Volume: 582 Pages: 71 - 89
ISSN: 1064-3745, 1940-6029 PMID: 19763943
DOI: 10.1007/978-1-60761-340-4_7
Publisher: Humana Press

Author:

Lipfert, Jan
Koster, Daniel A ; Vilfan, Igor D ; Hage, Susanne ; Dekker, Nynke H

Keywords:

DNA, DNA Breaks, Single-Stranded, DNA Topoisomerases, Type I, Humans, Magnetics, Microscopy, Nucleic Acid Conformation, Software, Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biochemistry & Molecular Biology, Single-molecule techniques, magnetic tweezers, topoisomerases, TopIB, spectroscopy, DNA TOPOISOMERASES, PERSISTENCE LENGTH, SUPERCOILED DNA, MICROMANIPULATION, ELASTICITY, Developmental Biology, 0601 Biochemistry and Cell Biology, 0399 Other Chemical Sciences

Abstract:

The past few years have seen the application of single-molecule force spectroscopy techniques to the study of topoisomerases. Magnetic tweezers are particularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomerases can be monitored using this technique.