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XIV National Academic Seminar of Biotechnology Students; IV International Students Conference of Biotechnology, Date: 2012/11/23 - 2012/11/25, Location: Gdansk

Publication date: 2012-11


Zownir, Marta
Bil, Magdalena ; Spibida, Marta ; Kreft, Lukasz ; Olszewski, Marcin




PriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and only three structures of them were resolved (Escherichia coli, Klebsiella pneumoniae and Neisseria gonorrhoeae). All up-to-date known PriB proteins have one OB domain per monomer and they are homodimers in solution.Recently, we have published the crystal structure of PriB protein from Thermoanaerobacter tengcongensis that represents new class of PriB with two oligonucleotide/oligosaccharide-binding domain (OB) per monomer that means it exists as monomer in solution. The aim of this study was identification of another bacteria with PriB proteins consist two OB domains. We analyzed above 2400 bacterial genomes searching for additional priB genes and we have found several bacteria from Clostridium class which contain priB gene coding PriB with two OB domains (e. g. Thermoanaerobacter tengcongensis, Clostridium tetani E88 ). Therefore, our studies suggest that we discovered new classes of PriB which weren’t published recently