Author:

Keywords:

03 Chemical Sciences, 06 Biological Sciences, 11 Medical and Health Sciences, Medicinal & Biomolecular Chemistry, 32 Biomedical and clinical sciences, 34 Chemical sciences, 42 Health sciences

Abstract:

An ethanolic extract of Taraxacum officinale Weber caused a dose-dependent inhibition of the ADP-induced aggregation, with a maximal inhibition of 85 ± 3.7%. A parallel dose-response curve, shifted towards three fold higher concentrations of extract, was obtained with higher ADP-concentrations. Arachidonic- and collagen-induced platelet aggregation was not influenced. A bioguided purification of the crude extract was performed. Purification included dialysis by means of Centricon®-10 concentrators. Since activity was recovered for > 95% in the lower Mr fraction (Mr < / 10.000), ADP-degrading apyrase could be excluded. Further purification was performed with gel permeation (Sephadex® G-50 superfine) and anion-exchange (SAX, triethylaminopropyl) chromatography. Activity was recovered in two fractions, one fraction contained low Mr (< / 10.000) polysaccharides while the second active fraction was characterized by the presence of triterpenes/steroids.